Abstract
Planting healthy seeds and other inputs have an impact on seed quality, fungal invasion deteriorates the seed quality in soil and storage. Studies on chickpea carried out at Seed Research and Technology Center, Rajendranagar, Hyderabad during spring 2010-11 2011-12 revealed that benomyl was found effective followed by Pseudomonas fluorescens in the inhibition of mycelial growth of Fusarium solani. Maximum germination percentage was recorded with Benomyl (Benomyl 500 WP) @ 2 g/kg seed (treated check) and Tebuconazole @ 1 ml/ kg seed (95 per cent) followed by treatment with P. fluorescens @ 10 g/kg seed along with soil application of P. fluorescens @ 3 kg/acre (94 per cent). Maximization of growth parameters like root length, shoot length and total seedling length were observed with Benomyl @ 2 g/ kg seed as 17.0 cm, 10.3 cm and 27.3 cm, respectively. Considering seedling vigour index as an important seed quality character, P. fluorescens and Benomyl @ 2 g/kg seed recorded high seedling vigor index. The per cent recovery of infested seeds was found to be low with treated seeds when compared to the control.
Highlights
Chickpea is the most nutritive pulse among different food legumes being extensively used as protein adjunct to starchy diet
Seed quality of chickpea was affected by incidence of seed borne diseases like wilt and root rot
MATERIAL AND METHODS Preliminary studies on seed mycoflora of chickpea were carried out under laboratory conditions and their inhibition was tested with four fungicides viz., Azoxystrobin, Tebuconazole, Carbendazim and benomyl and three bio-agents ie., Trichoderma viride, Bacillus subtilis and Pseudomonas fluorescence by rolled towel and seed inoculation technique
Summary
Chickpea is the most nutritive pulse among different food legumes being extensively used as protein adjunct to starchy diet. MATERIAL AND METHODS Preliminary studies on seed mycoflora of chickpea were carried out under laboratory conditions and their inhibition was tested with four fungicides viz., Azoxystrobin, Tebuconazole, Carbendazim and benomyl and three bio-agents ie., Trichoderma viride, Bacillus subtilis and Pseudomonas fluorescence by rolled towel and seed inoculation technique. In-vitro Evaluation of Bio-agents by Rolled Paper Towel Method: The powder formulation of antagonist’s viz., Trichoderma viride, Bacillus subtilis and Pseudomonas fluorescence were used for seed treatment to test their efficacy in overcoming seed borne fungal infection of chickpea under in-vitro conditions by rolled towel method. Benomyl was more effective in maximum germination and minimum seed borne infestation (92 and 16) followed by Pseudomonas ( 84 and 18) These results are in agreement in the inhibition of mycelial growth of Fusarium solani. These results are in agreement with the findings of Pankaj Sharma (2010) where the seed borne mycoflora was effectively controlled by fungicides
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