Potential water-based extracts from fresh and dried auricularia auricula-judaeon the inhibitory effect of human papillomavirus type 16 and cervical cancer cell line in caski

Abstract

Auricularia auricula-judae, also called the wood ear mushroom, is a well-known edible mushroom in various countries. Notably, the extracts of this mushroom inhibited the growth and expression of human papillomavirus (HPV) oncogene in the HPV-positive cervical cancer cell line. However, there has been no reported comparison of the effect of the extracts from fresh and dried A. auricula-judae on anti-HPV16 in CaSki cell lines. Thus, this study investigated the effect of the water-based extracts from fresh and dried A. auricula-judae at 100oC on anti-HPV16 pseudovirus infection and anti-cancer in CaSki as well as the expression of viral oncogenes and cellular genes. HPV16 pseudovirus was produced and used for anti-viral infection assay in pre-attachment and adsorption steps in treated 293FT cells. Cell apoptosis, cell proliferation, cell migration and colony-forming assay were investigated in treated CaSki cells. In addition, mRNA expression levels of HPV16E6/E7, BCL2, BAK and CASP3 were determined using quantitative real time polymerase chain reaction. The results indicated that the extract from dried mushroom caused higher cytotoxicity than fresh mushroom. Notably, the extracts from the dried mushroom more effectively inhibited the infection of HPV16 pseudovirus in 293FT cells than did fresh mushroom. Furthermore, it significantly decreased HPV16E6/E7 and BCL2 and increased BAK and CASP3 expression, in contrast to the untreated and water-treated CaSki cells, but not in the extract derived from fresh mushroom. The main conclusion was that the extract from dried mushroom more effectively inhibited growth of CaSki and HPV16-pseudovirus infection than from fresh mushroom.