Abstract
Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates of each serotype were analyzed for the identification of similar and different profiles. Dendrograms were constructed from molecular profiles using the UPGMA method (unweighted pair-group method for the arithmetic averages) and the software program WinBoot. The present study indicates the usefulness of RISA and ARDRA of the 16S-23S rRNA intergenic spacer region (ISR) for systematic, epidemiological, and diagnostic purposes. Since these techniques can be used for the differentiation of serotypes, they are highly promising for the characterization of Salmonella serotypes and intra-serotypes. Data indicate that these techniques may be used to produce more consistent, reliable, and reproducible results in the identification and epidemiological study (traceability) of Salmonella in the poultry industry.
Highlights
Salmonellosis is one of the most common infectious diseases in the world and affects both animals and humans
The purpose of the present study was to evaluate the usefulness and the potential application of different techniques based on PCR analysis (RAPD, ERIC, rDNA internal spacer analysis (RISA) and amplified ribosomal DNA restriction analysis (ARDRA)) for the differentiation of 15 Salmonella
The Salmonella isolates used in this study were obtained from poultry production chain environments, including hatcheries, broiler breeder farms, broiler farms and slaughterhouses located in the states of Santa Catarina and Rio Grande do Sul, between 2006 and 2010
Summary
Salmonellosis is one of the most common infectious diseases in the world and affects both animals and humans. About 2,600 Salmonella serovars are known and new serovars, which may potentially be foodborne pathogens, have been discovered (Guibourdenche et al, 2010). This high number and wide diversity of serovars causes the nomenclature of the Salmonella genus, species and serovars to be very complex (Smith et al, 2011). Enteritidis in broiler breeder (57.5%) and broiler flocks (84.0%), and it is the serovar most frequently responsible for foodborne outbreaks and sporadic cases of salmonellosis in humans (Kanashiro et al, 2005)
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