Abstract
Rice is an important cereal worldwide and it occupies the top position among the cereals in Bangladesh. Rice plant suffers from around 32 diseases of which ten are major in Bangladesh at present. Among the diseases, Bacterial Blight (BB) caused by X. oryzae pv. oryzae (Xoo) considered as a most destructive disease occurs in both rainfed and irrigated seasons of Bangladesh. BB causes considerable yield loss varies from 30 to 50% depending on the outbreak. It is also an important disease in most of the South and Southeast Asian countries. To develop environment-friendly sustainable management approach against BB of rice, in total sixty three plant growth promoting bacteria were identified from rice phylloplane and rhizosphere that are antagonistic to X. oryzae pv. oryzae during boro and aman seasons 2018 and 2019.These bacterial species inhibited the growth of X. oryzae pv. oryzae in vitro by 20.83 to 76.19%. These bacterial isolates were identified by sequencing of PCR products of 16SrDNA belonging to the genera mostly Pseudomonas, Bacillus and Serratia. Out of these bacterial species, 48 bacterial species were found as positive for IAA production, all 63 bacterial species were found positive for siderophore production and 48 were found capable to solubilize insoluble phosphate. Based on growth inhibition of X. oryzae pv. oryzae in in vitro, thirty two bacterial species were selected for plant growth promotion assessment and evaluation of net house and field efficacy in controlling BB of rice. These bacterial species were formulated using talcum powder which was viable for at least three months post formulation. Assessment of plant growth promoting determinants revealed that all 32 bacterial species identified in this study enhance the growth of rice plants as measured by root and shoot length and and reduced the BB severity in susceptible rice cultivar significantly as compared with untreated control.
Highlights
Rice (O. sativa L.) suffers from 32 diseases of which in Bangladesh 10 has been known as dreadful diseases [1]
Seventeen bacterial isolates were identified as antagonist against X. oryzae pv. oryzae and inhibited the growth of X. oryzae pv. oryzae in vitro which was ranged by 38.33–60.66% (Table 2)
The highest (60.66%) growth inhibition of X. oryzae pv. oryzae was exhibited by BDISO147Pand the lowest (38.33%) growth inhibition was shown by BDISO135P.These antagonistic bacterial isolates were identified by sequencing of PCR products of 16SrDNA gene (Figure 3B)
Summary
Rice (O. sativa L.) suffers from 32 diseases of which in Bangladesh 10 has been known as dreadful diseases [1]. Among the diseases three bacterial diseases are frequently occurred in Bangladesh. Among these three diseases, Bacterial Blight (BB) caused by X. oryzae pv. Oryzae (Xoo) considered as a most destructive disease occurs in all Agro Ecological Zones (AEZs) of Bangladesh and mostly in two rice growing seasons namely viz. In Japan, India and Bangladesh due to this devastating disease around 50%, 60% and 30% yield loss was observed [5], respectively in the highly infected rice fields. It is a crucial disease in most of the South and Southeast Asian countries [6]. In Bangladesh different pathogenic [13, 14] and genetic variability [15] have been detected and those were excessively perilous for rice [16]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call