Abstract
Proteeae Isolation Medium of Hawkey et al. (1986) was effective for isolation and enumeration of Proteus-Providencia-Morganella spp. from beef and beef products. On this medium colonies were brown, surrounded by a zone of clearing. In an examination of retail beef samples packaged and stored in polyvinyl chloride (PVC) film, 5% contained these bacteria in a concentration of 10 1–10 2 cells per gram or cm 2. Isolates from retail beef samples included P. vulgaris, P. alcalifaciens, P. rettgeri, and M. morganii. None of the retail samples packaged and stored in high oxygen barrier (HOB) film contained detectable Proteus-Providencia-Morganella spp. Steaks inoculated with Proteus-Providencia-Morganella spp. and packaged and stored at 4°C in PVC film (15 days) or in HOB film (42 days) usually had a reduced count of Proteus-Providencia-Morganella spp. during storage. Slight increases in counts occurred on steaks inculated with P. vulgaris and P. rettgeri and stored at 4°C for 15 days in PVC film.
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