Abstract

Diatoms are very diverse and highly productive organisms, found in a wide variety of environments. This study aims to analyze the growth and lipid composition of Phaeodactylum tricornutum, cultured in an outdoor pilot-scale bubble column photobioreactor under natural conditions in Chile for biodiesel production. Results showed that P. tricornutum cultures reached their highest biomass concentration (0.96 ± 0.04 kg m−3) after 14 days of culturing, at the stationary phase, with a volumetric productivity of 0.13 kg m−3 d−1. Biomass samples showed a total lipid content of 9.08 ± 0.38 wt %. The fatty acid methyl ester analysis revealed a composition of 24.39% C16-C18 fatty acids, 42.34% saturated fatty acids, 21.91% monounsaturated fatty acids and 31.41% polyunsaturated fatty acids. These findings suggest that P. tricornutum oil can be used as an alternative raw material for the production of biodiesel capable of meeting international quality standards.

Highlights

  • The production of biofuels offers new opportunities to mitigate climate change and promote energy security

  • The microalgae culture was performed with complete nutritional medium medium from the starting point, without further addition of nutrients

  • P. tricornutum was grown in the commercial culture medium f/2

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Summary

Introduction

The production of biofuels offers new opportunities to mitigate climate change and promote energy security. The complementation of fossil fuels, used for many decades as a primary energy source, leads to a circular economy approach by closing the carbon cycle [1]. In this context, microalgae have attracted significant interest as feedstock for different types of renewable fuels such as biodiesel, methane, hydrogen and ethanol, among others [2]. Microalgae photosynthetic metabolism produces valuable compounds using solar energy, water, carbon dioxide and other available compounds. Through this metabolic process, they are capable of generating raw materials to use in biofuels, food and feed production [3]. It is possible to effectively combine microalgae cultivation with wastewater treatment, reducing operating costs and simultaneously allowing potential waste valorization [6]

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