Abstract
The tomato pinworm, Tuta absoluta (Meyrick) (Gelechiidae: Lepidoptera), is an introduced serious pest of tomato in India. Management of this insect pest mainly relies on insecticides because of its high infestation levels on all plant parts and life stages of tomato crop. This laboratory study investigated the efficacy of Cry1Ac protein of Bacillus thuringiensis against T. absoluta. The LC50 and LC95 values for 2nd, 3rd, and 4th larval instars were 0.12, 0.27, and 0.43 μg/ml and 0.63, 0.71, and 2.64 μg/ml, respectively. Experimental results showed that Cry1Ac is effective against different larval instars of tomato pinworm.
Highlights
The tomato pinworm (TPW), Tuta absoluta (Meyrick) (Gelechiidae: Lepidoptera), is an important pest of tomato in India
Materials and methods Purified Cry1Ac toxin was obtained from the Centre for Plant Molecular Biology and Biotechnology (CPMB& B), Tamil Nadu Agricultural University (TNAU), Coimbatore, India
Based on the present study, it is evident that all the larval instars of the pest were susceptible to the Cry1Ac of Bacillus thuringiensis (Bt)
Summary
The tomato pinworm (TPW), Tuta absoluta (Meyrick) (Gelechiidae: Lepidoptera), is an important pest of tomato in India. It is a micro-lepidopteran and oligophagous pest native of South America. Tomatoes are grown both under greenhouse and open field conditions. One alternative to insecticides is the use of biological insecticides like Bacillus thuringiensis (Bt) that expresses insecticidal crystal (CRY) proteins during sporulation phase of its growth cycle These crystal proteins, which are sequestered in bacteria as crystalline inclusions, mediate specific pathogenicity against insects. Bt. Jalapathi et al Egyptian Journal of Biological Pest Control (2020) 30:81 formulates are very effective against T. absoluta under laboratory, greenhouse, and field conditions (Ghazwan et al, 2017). Corrected mortality percentages were worked out, using Abbott’s formula (Abbott, 1925), and subjected to probit analysis (Finney, 1971) from EPA Probit Analysis Program (version 1.5)
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