Potential investigation of anti-inflammatory and anti-oxidative property of ethanolic extract of Ixora nigricans leaves

Abstract

Our present study was intended to unleash pharmacological activity like cytotoxic, anti-oxidant and anti-inflammatory activity of Ixora nigricans (Family: Rubeacea). In ethnomedicine root extract of I. nigricans is taken for the treatment of diarrhea and leaf extract is for the treatment of dysentery. The ethanolic extract of Ixora nigricans leaves was studied using 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and quantitative analysis of anti-oxidative compound like flavonoids were made to estimate or to identify its total anti-oxidant property by using spectrophotometric method. In vitro anti-inflammatory activity was evaluated using membrane stability and protein (albumin) denaturation assay at different concentration. The DPPH scavenging result was 131.432.03g/ml and anti-oxidative compounds most importantly flavonoid content was 84.030.78 and total phenolic content was found 20.801.44. The anti-inflammatory activity by human RBC membrane stabilizing methods resulted 81.742.54% at 1000g concentration where as the compared standard (Aspirin) results 90.231.48% and in protein denaturation method it resulted 78.072.4% which was also close to Aspirin (86.281.48%). From these result it can be concluded that this has quite promising result and that may be due presence of flavonoids and some phenolic content.