Antiviral potential of mangiferin against poliovirus

Daniele Zendrini Rechenchoski,Nágila Maria Pontes Silva Ricardo,Ligia Carla Faccin-Galhardi,Carlos Nozawa,Arcelina Pacheco Cunha,Rosa Elisa Carvalho Linhares

doi:10.7439/ijpr.v8i4.4706

Abstract

Poliovirus (PV),an enterovirus of the Picornaviridae family, is the agent of poliomyelitis, a devastating neurologic disease that may result in paralysis and even death.Mangiferin is the major constituent of Mangifera indica and has multiple pharmacological activities, including antiviral. This study evaluated the activity of mangiferin against poliovirus type-1 (PV-1). The compound exhibited the 50% cytotoxic concentration (CC50) > 2000 μg/mL in HEp-2 cell cultures, by the dimethylthiazolyl-diphenyltetrazolium bromide method (MTT). The 50% inhibitory concentration (IC50) was of 53.5 μg/mL, determined by the plaque reduction assay (PRA) with selectivity index (SI) > 37.4. We investigate the possible mechanism of action of mangiferin and a maximum inhibition of PV was found for virucidal and inhibition of adsorption assays, at all tested concentrations. The inhibition of viral protein synthesis was also demonstrated by immunofluorescence assay (IFA).These results suggested that mangiferin is an attractive candidate for the control of PV infection.

Highlights

Poliovirus (PV), the etiologic agent of poliomyelitis, an enterovirus of the Picornaviridae family, is a non-enveloped virus with a positive single-stranded RNA genome [1;2]
The mangiferin showed no morphological changes in HEp-2 cell up to the highest concentration tested and presented a CC50 higher than 2000 μg/mL, by MTT assay
For the other time-of-addition protocols, at 200 μg/mL, mangiferin presented VI greater than 50%, 74.7% and 66.3% for 4 h and 12 h post-infection times, respectively

Summary

Introduction

Poliovirus (PV), the etiologic agent of poliomyelitis, an enterovirus of the Picornaviridae family, is a non-enveloped virus with a positive single-stranded RNA genome [1;2]. Despite intensive efforts to eradicate the virus, it remains endemic in Afghanistan, Nigeria and Pakistan [5]

Methods

Results

Conclusion