Potential growth and control of Escherichia coli O157:H7 in soft hispanic type cheese

Abstract

This study examined growth and control of two enterohemorrhagic Escherichia coli serotype O157:H7 strains in a soft Hispanic type cheese (Queso Fresco). Cheese was made in the laboratory using a commercial procedure and after inoculation it was stored under vacuum at temperatures ranging from 8 to 30 °C. The minimum temperature that allowed growth of E. coli in the unmodified normal cheese (pH 6.6) was 10 °C. No growth of either strains was observed during a 2 month storage period at 8 °C. Accumulated data from the growth studies were used for development of models relating square root of 1 LT (LT = lag time) and specific growth rate, to temperature. The effect of selected antimicrobials (potassium sorbate, sodium benzoate, and sodium lactate) added to milk or cheese on the growth arid survival of these pathogens at various storage temperatures was also evaluated. Addition of sodium benzoate (0.3%) to cheese (pH 6.6) or addition of potassium sorbate (0.3%) to cheese (pH 6) made from milk acidified to pH 5.9 with propionic acid, had a significant impact on delaying or preventing growth of the pathogens.