Abstract
Modern Applications in Pharmacy & Pharmacology Potential Application of Raman Micro-Spectroscopy as an In vitro Drug Screening and Companion Diagnostic Tool for Clinical Application: Chemotherapeutic Drug Mechanism of Action, Cellular Effects and Resistance Z Farhane1,2* F Bonnier3 and H J Byrne1 1FOCAS Research Institute, Dublin Institute of Technology, Ireland 2School of Physics and clinical and optometric Sciences, Dublin Institute of Technology, Ireland 3Université François Rabelais de Tours, France *Corresponding author: Z Farhane, FOCAS Research Institute, School of Physics and clinical and optometric Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland Submission: October 31, 2017; Published: November 13, 2017 DOI: 10.31031/MAPP.2017.01.000506 ISSN 2637-7756Volume1 Issue2
Highlights
Traditional diagnostic methods are largely based on identification of morphological changes in cells or tissues, rather than analysis of the underlying biochemistry, and so are subjective and prone to error
Many studies have reported the use of vibrational spectroscopy to monitor the effects of anticancer agents on cancer cells, including, polyphenols [5] cardiotonic steroids [6], platinum compounds [7], epidermal growth factor inhibitor [8], gold based metallo drugs [9], plant alkaloids [10,11,12,13] and anthracyclines [14,15,16,17,18]
Using DOX, Raman micro-spectroscopy clearly demonstrates that it accumulates and saturates first the nucleoli, selectively targeting the RNA, the nucleus, before it accumulates in the cytoplasm, after nuclear disruption [19,20]
Summary
Traditional diagnostic methods are largely based on identification of morphological changes in cells or tissues, rather than analysis of the underlying biochemistry, and so are subjective and prone to error. As an in vitro molecular fingerprinting technique with optical resolution, Raman micro-spectroscopy is able to monitor biochemical processes, drug uptake, efficacy and mode of action and mechanisms of interaction of chemotherapeutic drugs at a sub cellular level and can help guide drug design
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