POTENT ANTIBACTERIAL OF GEL KAWANG FRUITS (Litocarpus celebicus, (Miq) Rehder) ETHANOL EXTRACT ORIGIN FROM PAPUA INDONESIA

Abstract

Objective: Kawang fruit (Lithocarpus celebicus (Miq.) Rehder) is native plant of Digul-Bouven, Papua, Indonesia have flavonoids which has antibacterial activity. Antiseptic is a substance used to inhibit the growth or destroy microorganisms that live on the surface of the body. Their resistance to antiseptics on the market, it needed a new antiseptic-based natural ingredients that are relatively safer. The purpose of this study to make an antiseptic gel with extract ethanol of kawang fruit and determine its effectiveness against Staphylococcus aureus ATCC 29 213 and Escherichia coli ATCC 25922.Methods: extraction, phytochemical screening, testing activities with the agar diffusion method, determination Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC), antiseptic gel formulation, and test preparation gel. The test results showed the kawang fruit has activity against bacteria with value MIC and MBC on Staphylococcus aureus ATCC 29213 is 5% (b/b) and 10% (b/b), and on Escherichia coli ATCC 25922 is 10% and 20%.The extraction is formulated in the form of a gel with a concentration of 5%, 10%, and 20%. Formulations made using 2% HPMC.Results: The test results show the effectiveness of antiseptic gel extracts have inhibitory better on Staphylococcus aureus ATCC 29213 than Escherichia coli ATCC 25922.Conclusion: Antiseptic gel preparation of kawang fruit ethanol extract at a concentration of 5% have shown their antibacterial effectivity. Its inhibition to Staphylococcus aureus ATCC 29213 was better than Escherichia coli ATCC 25 922.