Potency of mangosteen (Garcinia mangostana L.) pericarp on seminiferous tubules testes streptozotocin-induced diabetic rats

Abstract

The diversity of compounds in mangosteen peel (Garciniamangostana L.) is suspected to have hypoglycemic activity. The objective of this study to analyze the blood glucose level and seminiferous testicular tubules tissue structure of streptozotocin-induced diabetic rats. The experiment animals were twelve male wistar strain white rats (Rattus norvegicus), 2-3 months old, weighing 120-150 g, put into four different groups i.e. (1) negative control treatment (P0), (2) streptozotocin (STZ) induced intraperitoneal as much as 80 mg/kg in 0.1 M buffer citrate with positive control pH 4.5 (P1), (3) 83.3 mg/kg mangosteen pericarp powder (P2), and (4) 0.09 mg/kg glibenclamide (P3) for 36 days. The analysis of the blood glucose levels and seminiferous testicular tubules tissue structure using HE staining, identification with the optilab microscope. The study found that diabetic tubules testes structure experienced degeneration, the spermatogenic cells were not much identified, lumen tubules testes diameter 703,25 μm (P1), spermatogonia cells were identified, the spermatogenesis developed, lumen tubules testes diameter 570,53 μm (P2). The mangosteen solution and glibenclamide could lower the blood glucose level and improve the spermatogenesis of the diabetic rats. The mangosteen pericarp solution did not damage the renal tissue structure, unlike the glibenclamide. It was concluded that the mangosteen solution has the potential as natural anti-diabetic substance.