Potencial genotóxico del extracto oleoso de la semilla de Carapa guianensis Aublet para inducir anomalías en la morfología de la cabeza del espermatozoide

Abstract

ObjectiveThis study has aimed to evaluate the genotoxic potential of Carapa guianensis seed oil extract to induce sperm head abnormalities in Balb/c mice. Material and methodsFive experimental groups were formed: one placebo group (Tween65, 2%), three extract dose levels (400, 1000 and 2000mg/kg) administered orally for 35days and one positive control group treated with cyclophosphamide (CP) at a dose of 50mg/kg via intraperitoneally for 5 consecutive days and then 35days of rest after the last administration with the mutagen. Were distributed 8animals per group. The animals were euthanized by cervical dislocation 35days after the administration and the epididymis was extracted to proceed with the sperm head morphology assay. Results and discussionThere were no significant differences in the results between the controls and those treated with the extract regarding frequency of the different sperm head anomalies or in the spermatic concentration and sperms with cytoplasmic drop. However, a difference was found between the controls and treated versus the CP treated group, validating our results. We have concluded that the oleaginous extract of Carapa guianensis seeds does not have genotoxic potentialities to induce an increase in the spontaneous frequency in the sperm head morphology of male Balb/c mice.