The effect of He-Ne Laser and UVC irradiation on sperm head morphology and DNA of testis for albino mice(Mus musculus)

Abstract

In vitro, He-Ne laser show a modifying response of cells to ionizing radiations. So there is a need to investigate this property in vivo.To investigate the effect of He-Ne laser (632.8nm) pre and post irradiation against the UVC (253.7nm) irradiation in vivo on the sperm morphology, and DNA of testis in Mouse. In this study Twenty adult albino male mice were divided into four groups, the first group was He-Ne laser irradiated, and the second group was UVC irradiated. The third group was pre-irradiated by He-Ne laser followed by UVC irradiation, with one hour time interval between the two irradiations. The fourth group was pre-irradiated by UVC then by He-Ne laser, with one hour separating the two irradiations. The He-Ne laser per-irradiation show a protection properties, which appeared on both sperm head abnormality and DNA damage against UVC light irradiation. Four sperm head abnormality features were found after each type of irradiation. But the He-Ne laser preirradiation against UVC irradiation lower the frequencies of sperm head abnormality, farther more reduce the DNA testis damaging. Both UVC and He-Ne laser irradiations revealed a toxic effect on sperm head morphology, but the HeNe laser was found less effective. The He-Ne laser protective properties was observed only when He-Ne laser irradiation was applied before the UVC irradiation.