Potassium currents in primary cultured astrocytes from the rat corpus callosum

Abstract

The corpus callosum (CC) is the main white matter tract in the brain and is involved in interhemispheric communication. Using the whole-cell voltage-clamp technique, a study was made of K(+)-currents in primary cultured astrocytes from the CC of newborn rats. These cells were positive to glial fibrillary acidic protein after culturing in Dulbecco's Modified Eagle Medium (> 95% of cells) or in serum-free neurobasal medium with G5 supplement (> 99% of cells). Astrocytes cultured in either medium displayed similar voltage-activated ion currents. In 81% of astrocytes, the current had a transient component and a sustained component, which were blocked by 4-aminopyridine and tetraethylammonium, respectively; and both had a reversal potential of -66 mV, indicating that they were carried by K(+) ions. Based on the Ba(2+)-sensitivity and activation kinetics of the K(+)-current, two groups of astrocytes were discerned. One group (55% of cells) displayed a strong Ba(2+) blockade of the K(+)-current whose activation kinetics, time course of decay, and the current-voltage relationship were modified by Ba(2+). This current was greatly blocked (52%) by Ba(2+) in a voltage-dependent way. Another group (45% of cells) presented weak Ba(2+)-blockade, which was only blocked 24% by Ba(2+). The activation kinetics and time course of decay of this current component were unaffected by Ba(2+). These results may help to understand better the roles of voltage-activated K(+)-currents in astrocytes from the rat CC in particular and glial cells in general.