POSTN promotes granulosa cell proliferation in sheep follicles through focal adhesion

Abstract

BackgroundThe complex regulatory mechanisms involved in follicular development in sheep are not fully understood. This study aimed to understand the expression of periostin (POSTN) in granulosa cells (GCs) and its effect on follicle development. MethodsThe effect of luteinizing hormone (LH) treatment on POSTN expression in GCs was examined. POSTN was overexpressed or inhibited in GCs, and its effects on cell proliferation and apoptosis, as well as potential mechanisms of action, were evaluated. ResultsPOSTN showed higher expression in ovarian tissue than in other tissues and higher expression in large follicles than in small follicles. LH treated GCs promoted POSTN expression. Overexpression of POSTN in GCs significantly increased cell proliferation and inhibited apoptosis. Phosphorylation levels of focal adhesion kinase and autophagy were elevated in POSTN overexpressing GCs. ConclusionPOSTN affects GCs proliferation and follicle development through the focal adhesion, potentially improving fecundity in sheep.