Abstract
BackgroundVitrification has become the method of choice for cryopreservation of human embryos and gametes. There are multiple commercial media, containing different combinations and concentrations of cryoprotectants, available for vitrification and warming procedures. The aim of this retrospective study was to compare post-warming survival rate and clinical outcomes of cleavage stage embryos vitrified/warmed using two different commercial methods (CryoTouch and Cryotop) during intracytoplasmic sperm injection/frozen embryo transfer (ICSI/FET) cycles. This retrospective study evaluated a total of 173 FET cycles performed on 446 warmed cleavage stage embryos between January 2018 and December 2020. Post-warming embryo survival rate and clinical outcomes including clinical pregnancy, implantation, and live birth rates were calculated.ResultsThe results showed no significant differences between two groups in terms of post-warming survival rate (p value = 0.5020), clinical pregnancy rate (p value = 0.7411), implantation rate (p value = 0.4694), and live birth rate (p value = 0.5737).ConclusionsCollectively, high successful rates were observed in outcomes of vitrified/warmed cleavage stage embryos using both CryoTouch and Cryotop commercial methods.
Highlights
Vitrification has become the method of choice for cryopreservation of human embryos and gametes
Sazeh Co., Tehran, Iran) and Cryotop method (Kitazato BioPharma Co., Shizuoka, Japan). Study population This retrospective study was conducted to analyze the survival rate of cleavage stage embryos after vitrification/warming performed by CryoTouch and Cryotop methods, and the subsequent clinical outcomes in two infertility clinics located at Erfan Niayesh Hospital Tehran, Iran and Mehr Fertility Research Center, Rasht, Iran, from January 2018 to December 2020
A total of 978 Frozen embryo transfer (FET) cycles involving embryos vitrified/warmed on day 3 of development were evaluated to enter the study based on the following inclusion/exclusion criteria: The inclusion criteria were age range between 20 and 35 years old, body mass index (BMI)
Summary
Vitrification has become the method of choice for cryopreservation of human embryos and gametes. There are multiple commercial media, containing different combinations and concentrations of cryoprotectants, available for vitrification and warming procedures. The aim of this retrospective study was to compare post-warming survival rate and clinical outcomes of cleavage stage embryos vitrified/warmed using two different commercial methods (CryoTouch and Cryotop) during intracytoplasmic sperm injection/frozen embryo transfer (ICSI/FET) cycles. This retrospective study evaluated a total of 173 FET cycles performed on 446 warmed cleavage stage embryos between January 2018 and December 2020. Vitrification has been broadly used for human cleavage and blastocyst stage embryos [6, 11]
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