Abstract
The potential of algae biofuel production in conjunction with wastewater treatment is considered an alternative energy source, which is recently proposed. The scope of this study was to investigate the use of Chlorococcum sp. in wastewater treatment and the evaluation of algal biomass and lipid production. The experiments were conducted using a 30-L open pond. The pond was fed with primary and secondary treated wastewater from an activated sludge treatment plant. Experiments were conducted under controlled environmental conditions in order to investigate the removal of nutrients, biomass growth, and algal lipid content. The pond initialy treated secondary effluent at hydraulic retention time (HRT) from 2.5 to 6 days, and radiation intensity of 100 μmol m−2 s−1. With the primary effluent as substrate, the pond was operated at an HRT of 30 days, and radiation intensity of 100 and 200 μmol m−2 s−1. The growth rate of microalgae treating the secondary effluent was greater at low HRT, compared to higher HRTs. The highest biomass concentration was observed when the pond was treating the primary effluent. Nitrates removal with the secondary effluent was up to 98 %, and the lower removal efficiencies were observed at low HRTs. The higher lipid content of Chlorococcum sp. was observed when the pond was fed with the secondary effluent.
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