Post harvest mycobiota of sissoo(Dalbergia sissoo Roxb) grown in north Eastern U.P and their culture filterate potential

Abstract

Seed samples were collected from 25 places of north eastern U.P from luxuriantly growing and healthy D.sissoo Roxb plants and stored at room temperature for 3 months in presterilized polyethylene bags.Mycofloral analysis showed presence of 15 fungal species which showed variation at district level on the basis of occurence.Mycofloral analysis through blotter method revealed the presence of 15 fungal species and agar plate method 12 fungal species.Seven fungal species of three genera were detected from surface sterilized seeds using blotter method.Twelb fungal species(belonging to 6 genera) and 5 fungal species (of two genera) were detected from unsterilized and sterilized seeds from agar plate method of analysis.The metabolites of most of the fungi showed effect on germination and put A.niger as highly potent.The other fungi in order of potentials for inhibiting seed germination were F.solani,F.oxysporum,A.tamarri,A.terreus,F.moniliforme,A.phoenicis,A.flavus,Alternaria alternata,Aspergillus candidus,Penicillium glabrum,Rhizopus nigicans and Trichothecium roseum.The metabolite of A.sydowi and Trichoderma viride showed promotive effect on the germination of the seeds of sissoo.A.niger and F.solani caused high degree of inhibition in germination and also caused high degree of mortality.