Possibility of using embryonic neural cells to regenerate structures of the inner ear of Guinea pigs after experimental ototoxicosis

Abstract

Hearing disorders greatly impair the comfort of life, change a person's emotional state, and when developed in early childhood lead to disorders of psycho-social formation of personality. There are currently no effective treatments for patients with sensorineural hearing loss (SNHL). Therefore, all over the world prefer "substitution tactics", namely - hearing aids for deafness, or cochlear implant for deafness. Aim. To evaluate the influence of NEC in different ways of their introduction on the morphological state of the structures of the inner ear (Corti's organ and the first neuron, vascular strip), as well as the auditory nerve on the background of aminoglycoside ototoxicosis. To investigate the characteristics and directions of reorganization of hemoblood supply and features of cyto - and myeloarchitectonics of structures of the inner ear. Materials and methods. To study the effectiveness of neuronal embryonic cells (NEC) in aminoglycoside ototoxicosis, experimental studies were performed on 40 guinea pigs weighing from 500 to 600 g. SNHL was caused by the introduction of aminoglycoside antibiotic - gentamicin sulfate at a dose of 100 mg/kg for 14 days. The neuronal stem cell suspension was administered in a volume of 2 million cells in 0.5 ml intratympanically and 2 million cells in 0.5 ml suboccipitally on days 1 and 15 of the experiment. Results and discussion. The influence of NEC on different methods of their introduction on the morphological state of the structures of the inner ear (Corti's organ and the first neuron), as well as the auditory nerve on the background of aminoglycoside ototoxicosis was evaluated. The characteristics and directions of reorganization of hemoblood supply and features of cyto - and myeloarchitectonics of structures of an inner ear according to an estimation of neurovasal relations are investigated. Conclusions. The data obtained indicate that intratympanic and suboccipital administration of NEC promotes the regeneration of damaged cell structures of the inner ear. This opens the prospect of using NEC to develop new approaches in the treatment of patients with sensorineural hearing loss.