POSSIBILITIES OF APPLICATION OF LASER POLARIZATION METHODS FOR THE DETECTION OF PATHOLOGICAL PROCESSES IN THE LIVER TISSUE WITH TEMPORARY MONITORING OF THE DYNAMICS OF CHANGES DEPENDING ON THE TIME SINCE DEATH

Abstract

The article presents the current state of issues regarding the diagnosis of liver fibrosing processes. Particular attention is paid to biophysical research methods that have evidence of accuracy, objectivity, reproducibility of results.Aim of the work. Exploring the possibilities of using azimuthal-invariant Mueller-matrix analysis to establish the relationship between changes in the morphological structure of liver tissue and its optical parameters in normal and fi brosing processes.Materials and methods. The object of the study were histological sections of human liver in normal (control group) and in the presence of fibrosing processes, selected from 106 corpses of both sexes at diff erent intervals after death. Histological sections of the liver were obtained using a freezing microtome. The studies were performed using a standard spectropolarimeter scheme. The obtained results were processed according to standard algorithms of MATLAB and Statistica software products.Results. According to the results of the study, it was found that in the liver tissue with fibrous changes, the values of the average S1 are (1,870±0,017), dispersion S2 – (0,617±0,010), asymmetry S3 – (1,921±0,022), excess S4 – (2,005±0,044). In intact liver tissue, the values were: S1 – (1,264±0,017), S2 – (0,537±0,018), S3 – (1,650±0,022), S4 – (1,584±0,032).Conclusion. The method of azimuthal-invariant Mueller-matrix analysis allows to describe in detail the structure and characteristics of biological tissues of the human body, in particular the liver in normal and fi brosing processes, with the possibility of their statistically signifi cant differentiation.The interval of dynamics of change of statistical moments of the 1st-4th orders from 1 to 48 hours after death is established that becomes extremely actual in practice of the forensic expert at the decision of important questions.