Effect of Fenugreek Seed Extract on Some Haematological and Biochemical Parameters in Atrazine Treated Male Rats

Abstract

Atrazine (ATR) is one of the most commonly used herbicide. The present study designed to investigate the effect of boiled aqueous extract of 2.5% and 5% of fenugreek seeds in the diet with 150 mg ATR/kg body weight (B.W.), on B.W. , food intake, some haematological and lipid profile parameters, and histological sections of liver and kidney in male albino rats for 4 weeks . The animals were divided equally and randomly into four groups including ATR , ATR+2.5% fenugreek , ATR+5% fenugreek and control group. The animals B.W. in weeks 2 and 3 was decreased significantly in ATR group when compared to control , while it was decreased non significantly in all weeks of ATR+2.5% fenugreek and significantly in week 2 in ATR+5% fenugreek from ATR group. Food intake in ATR group was decreased in all weeks, while it was changed non significantly in ATR+2.5% and ATR+5% fenugreek from ATR group . Non-significant change occurred in haematological parameters of all experimental groups. Total cholesterol in ATR+2.5% fenugreek and in ATR+5% fenugreek were reduced non significantly from ATR group . Meanwhile, cholesterol was increased non significantly in ATR+2.5% fenugreek and significantly in ATR+5% fenugreek from control. Triglyceride TG and LDL in ATR group were increased non significantly from control. While in ATR+2.5% fenugreek and ATR+5% fenugreek they were reduced non significantly from ATR group. HDL was decreased non significantly from control. While in ATR + 2.5% fenugreek was increased significantly from ATR and non significantly from control .Whereas in ATR + 5% fenugreek was increased non significantly from ATR and control as well. Fenugreek decreased degeneration in liver and kidney sections, however the nuclei of some cells in ATR+5% fenugreek were recognized with dark color . In conclusion the present study suggested that fenugreek reduce ATR toxicity, and the aqueous extract of 2.5% of fenugreek is preferred on 5% against ATR.