Positive association of -420C > G single nucleotide polymorphism in resistin gene promoter with insulin resistance indices in diabetic type 2 patients

Abstract

IntroductionResistin is considered as an important adipocytokine that may have an association with obesity and insulin resistance. The association of resistin gene polymorphism with type 2 diabetes has been proved. In this study, we aim to investigate the association between polymorphism (rs1862513 RETN) of the promoter resistin gene and susceptibility to type 2 diabetes in Zanjan province of Iran. Methods and materialsOne hundred seventy five patients with type 2 diabetes and 175 non-diabetic individuals were participated in this case-control study. The DNA was extracted from the whole blood. Genotyping was performed using Restriction Fragment Length Polymorphism (RFLP)-PCR method technique for all individuals. The relationship between genotypes, clinical and biochemical variables such as age, fasting blood sugar and blood pressure (BP) were evaluated by automatic enzymatic method. ResultsThe current study showed that the genotype distribution of resistin gene promoter variant was different between diabetic and nondiabetic groups. The frequency of GG genotype in diabetes patients and non-diabetic subjects was 17.1% and 9.7%, respectively which considered this genotype as significant (OR = 2.168, CI = 1.106–4.252 and P = 0.024). The present study showed this polymorphism may contribute to diabetes susceptibility. ConclusionMultivariate Logistic regression models by considering confounding showed that variables such as high-density lipoprotein, Body mass index (BMI), sex and age has greater predictive validity than GG genotype. In addition, more studies with greater sample sizes is required to confirm our results.