Abstract
Asini Corii Colla (ACC; donkey-hide glue) is one of the most valuable tonic traditional Chinese medicines. Because of the large demand for gelatinous Chinese medicines, bovine or swine skin was sometimes used to make adulterated gelatine in recent decades. Food chemicals can greatly harm people’s health, and detecting chemicals in foods is extremely important. A loop-mediated isothermal amplification (LAMP) device with smartphone detection is demonstrated in this study for detecting the DNA of Asini Corii Colla. The complete system is composed of a hand-held box equipped with a smartphone, a cartridge heater, an ultraviolet LED, a disposable reaction tube, and a homemade thermal module. All the processes are powered by a set of rechargeable batteries. Comprehensive experiments of measuring temperature profiles are presented, which showed the accuracy of temperature under thermal control is less than 0.5 °C. By implementing one heating module with an ATmega328p-au microcontroller in the device, the DNA mixture is heated directly up to the reaction temperature within 5 min. Next, a DNA segment of Asini Corii Colla is utilized to evaluate the sensitivity of the DNA amplification in the portable device. A limit of detection to a concentration of 10−4 ng/μL is achieved. Real-time detection of Asini Corii Colla by a smartphone camera can be achieved using this portable device. The unique architecture utilized in this device is ideal for a low-cost DNA analysis system.
Highlights
Food security is one of the main concerns in modern society in the twenty-first century
The loop-mediated isothermal amplification (LAMP) experiments on deoxyribonucleic acid (DNA) samples extracted from Asini Corii Colla were carried out, and real-time detection by a smartphone was implemented in the portable device
The performance of LAMP amplification of the portable device was compared to the conventional method
Summary
Food security is one of the main concerns in modern society in the twenty-first century. People generally eat food to avoid hunger. Some chemicals in food ingredients are allowed within strict limitations, a heavy penalty, legislated by the various governments, is applied to those people or companies that disobey the policies. Sometimes people eat specific food just from their desire to stay healthy. Certain health foods are announced to be good for the human body and the price becomes very high. Dishonest merchants make fake health food and sell it in public markets to earn money. Fake health food may cause no definite harm to human bodies, but some can cause health problems and/or severe harm to certain people
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