Rapid and Sensitive Diagnosis of Porcine Parvovirus by Loop- mediated Isothermal Amplification (LAMP) Method

Abstract

Qu, G., Fu, S., Shen, N., Wang, J., Xiao, Y., Guan, Y., Tang, N., Chen, L., Gao, S. and Shen, Z. 2010. Rapid and sensitive diagnosis of porcine parvovirus by loop-mediated isothermal amplification (LAMP) method. J. Appl. Anim. Res., 37: 113–116. To form a detection model for the porcine parvovirus (PPV), six special primers were designed based on the PPV gene NS1. After optimization only 45 min were needed to amplify PPV DNA by incubation at 63C. The specificity of the LAMP assay was validated by the absence of any cross-reaction with six other genetically related viruses, followed by restriction digestion and was observed to be similar to PCR assay. The LAMP assay demonstrated 100-fold higher sensitivity compared to PCR, with a detection limit of 12fg DNA. Thus the LAMP can be well-applied to laboratories, as a portable device and valuable tool for differential diagnosis of PPV in the countryside.