Abstract
Porcine Epidemic Diarrhea Virus Variants with High Pathogenicity, China
Highlights
Sequences for the S gene were submitted to GenBank
The piglets were randomly allotted to 3 groups, each group consisting of 5 pigs. These piglets were shown by serologic analysis to be negative for antibodies against porcine epidemic diarrhea virus (PEDV), porcine reproductive and respiratory syndrome virus, porcine transmissible gastroenteritis virus, and pseudorabies virus
PEDV was recovered from the dead piglets, and the full-length S gene was amplified by reverse transcription PCR and sequenced
Summary
Sequences for the S gene were submitted to GenBank (accession nos. for YS: JQ771753; SH: JQ771751; and ZB: JQ771752). To determine the virulence of the PEDV isolates, we experimentally infected fifteen 4-day-old Duroc crossbred piglets with the YS and ZB These piglets were shown by serologic analysis to be negative for antibodies against PEDV, porcine reproductive and respiratory syndrome virus, porcine transmissible gastroenteritis virus, and pseudorabies virus. In the piglets inoculated orally with 1.5 mL of YS isolate (103.0 50% tissue culture infectious doses/mL), diarrhea was observed at [3,4,5,6] days postinfection (dpi).
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