Abstract

Isolation of Porcine Epidemic Diarrhea Virus during Outbreaks in South Korea, 2013-2014.

Highlights

  • To the Editor: Porcine epidemic diarrhea (PED) is an acute infectious diarrhea caused by the PED virus (PEDV), which belongs to the order Nidovirales, family Coronaviridae, genus Alphacoronavirus (1)

  • One field strain of PEDV was successfully adapted for growth on Vero cells. This virus was isolated from a 60sow farm that had not vaccinated its animals against PEDV

  • Examination at necropsy revealed that the dead piglets from BM farm were covered with brown blotches of dried diarrheal feces and their stomachs were filled with undigested milk

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Summary

Introduction

To the Editor: Porcine epidemic diarrhea (PED) is an acute infectious diarrhea caused by the PED virus (PEDV), which belongs to the order Nidovirales, family Coronaviridae, genus Alphacoronavirus (1). Attempts to isolate the field strains of PEDV on Vero cell lines followed a previously described protocol with modifications (6). One field strain of PEDV (named BM1) was successfully adapted for growth on Vero cells. Translucent small intestines that contained yellow fluid were observed (online Technical Appendix Figure 1, http:// wwwnc.cdc.gov/EID/article/21/12/15-0437-Techapp.pdf).

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