Abstract
Porcine coronavirus HKU15 detected in 9 US states, 2014.
Highlights
Between February 7, 2014, when porcine coronavirus (PorCoV) HKU15-OH1987 was first identified in Ohio [2], and April 9, 2014, the Animal Disease Diagnostic Laboratory of the Ohio Department of Agriculture received >2,000 swine samples from farms in 10 US states for diagnosis of diarrheal disease
To determine the genetic diversity of PorCoV HKU15 strains from the 9 states, we conducted wholegenome sequencing for 1 strain from each state by using 16 pairs of previously described, overlapping primers [2]
Sequence analysis showed that strains from South Dakota (SD3424), Nebraska (NE3579), Illinois (IL2768), Indiana (IN2847), Kentucky (KY4813), Michigan (MI6148), and Pennsylvania (PA3148) have the same genome size (25,422 nt) as OH1987, and wholegenome pairwise comparison showed that they share a high nucleotide similarity (>99.8%)
Summary
To determine the genetic diversity of PorCoV HKU15 strains from the 9 states, we conducted wholegenome sequencing for 1 strain from each state by using 16 pairs of previously described, overlapping primers [2]. Strain names were designated by the state abbreviation and case number. The complete sequence for HKU15-OH1987 was reported previously [2]. Sequence analysis showed that strains from South Dakota (SD3424), Nebraska (NE3579), Illinois (IL2768), Indiana (IN2847), Kentucky (KY4813), Michigan (MI6148), and Pennsylvania (PA3148) have the same genome size (25,422 nt) as OH1987, and wholegenome pairwise comparison showed that they share a high nucleotide similarity (>99.8%).
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