Populational divergence in the impact of three nitrogenous compounds and their combination on larvae of the frog Pelophylax perezi (Seoane, 1885)

Abstract

Pollution by nitrogenous compounds is a putative stressful factor that may be causally linked to the decline of amphibians. One way to understand the potentially detrimental consequences of eutrophication on amphibian populations is to investigate variation among populations differing in exposure to nitrogen, this variation potentially indicating evolutionary potential to cope with this stressor. We have examined the effect of nitrogenous compounds ( NH 4 + ; NO 2 - ; NO 3 - , both alone and in combination) on fitness-related larval traits in four populations of Pelophylax perezi naturally exposed to different degrees of eutrophication. The results indicate that both survival and larval final size decrease at higher concentrations of these compounds, either singly or in combination. Additionally, the nitrogenous compounds were more lethal and larval food consumption and final mass were significantly reduced when they were exposed to combinations of compounds. Populations inhabiting highly polluted aquatic environments tolerated higher levels of nitrogenous compounds and showed higher survival rates and larger final size than the populations of less polluted environments, suggesting the potential to adapt to increased nitrogenous contamination in this species.