Population trends in the fine spatial re-organization of synaptic elements in forebrain regions of chicks 0.5 and 24 hours after passive avoidance training.

Abstract

Two regions in the forebrain of domestic chicks (Gallus domesticus), the intermediate and medial hyperstriatum ventrale and the lobus parolfactorius, have previously been shown to be important centres of biochemical, pharmacological and physiological change following one-trial passive avoidance training. The purpose of the present study was to examine, at the electron microscopic level, the fine spatial re-arrangement of synaptic structures in the intermediate and medial hyperstriatum ventrale (at 30 min), and in the lobus parolfactorius (at 24 h), post-training using comprehensive biometrical designs, image analysis and stochastic approaches. In intermediate and medial hyperstriatum ventrale, no significant differences in the numerical density of synapses either between control and trained chicks, or between hemispheres, were revealed using the disector method. However, after training, a nested-ANOVA demonstrated an increase in the thickness of pre- and post-synaptic electron densities (estimated via image analysis) only in the left intermediate and medial hyperstriatum ventrale, whereas synaptic apposition zone profiles increased in length bilaterally. In presynaptic terminals from the intermediate and medial hyperstriatum ventrale, stochastic analysis revealed that training resulted in the re-distribution of synaptic vesicles between two spatial pools relative to synaptic apposition zones, in both hemispheres producing a large number of synaptic vesicles closer to synaptic apposition zones; a nearest neighbour analysis of synaptic apposition zone profiles indicated that the lateral shape of the synaptic apposition zone after training is more complex in both hemispheres. In the lobus parolfactorius at 24 h post-training the main changes in synaptic fine structure involved a shift of synaptic vesicles away from synaptic apposition zones in the right hemisphere with the distance between synaptic apposition zones decreasing; in the left lobus parolfactorius, synaptic apposition zones became more regular/round in shape with a greater distance between them after training. These data suggest that the initial acquisition of memory involves population changes in the fine spatial organization of synaptic vesicles and synaptic apposition zones in synapses in the intermediate and medial hyperstriatum ventrale, which indicate a possible tendency towards greater synaptic efficacies. These changes are as dynamics as the molecular changes which have hitherto been considered the preserve of short-term correlates of memory formation.