Population-level variation in neonicotinoid tolerance in nymphs of the Heptageniidae

Abstract

Anthropogenic activities can have significant ecological and evolutionary consequences on populations and communities. In the United States, neonicotinoid insecticides are widespread across the agricultural Midwest and frequently detected in stream systems. Their effect on Heptageniidae mayflies is a major concern because they are highly sensitive to neonicotinoids and have some of the lowest reported tolerance values of any organism. Our objective was to evaluate population-level variation in neonicotinoid sensitivity. We did so by conducting 96 h half maximal effective concentration (EC5096-h) tests for the neonicotinoids clothianidin and thiamethoxam on populations of Stenacron, Stenonema, and Maccaffertium mayflies and testing for associations with agricultural landcover. Additionally, we collected water samples to assess temporal patterns of neonicotinoid presence in stream habitats. We found variation in neonicotinoid tolerance with EC50 values ranging from 4.9 μg/L to 32 μg/L and 19.8 μg/L to 86.5 μg/L for clothianidin and thiamethoxam, respectively. Agricultural landcover was associated with neonicotinoid tolerance for Stenacron and thiamethoxam but not for other comparisons. Moreover, water samples demonstrated that the amount of agricultural landcover was not a strong predictor of neonicotinoids presence in streams. Our data suggest that populations of Heptageniidae mayflies can vary substantially in neonicotinoid tolerance. Population-level variation should be considered in toxicity assessments and presents the potential for evolved tolerance.