Population genetic data for F13A01, FES/FPS, F13B and LPL in the south Portuguese population

Abstract

DNA parentage testing is currently performed using several highly polymorphic short tandem repeats (STRs). In our routine casework, we apply two validated STRs kits, in order to have results in the 13 codis loci plus D2S1338, D19S433, PENTA E, PENTA D and Amelogenin. In complex and deficient paternity cases it is often necessary to increment the number of studied STRs. For this reason, we introduced in our laboratory GenePrint ® FFFL Multiplex kit, which can provide results in F13A1, FES/FPS, F13B and LPL using the GenePrint ® FFFL System (Promega, USA) kit. In this study we analysed 150 unrelated and healthy individuals from south Portugal population. Allele frequencies and statistical parameters were estimated with Arlequin 3.5.1.2. Paternity Statistics were calculated using software package PowerStats v12. The forensic efficiency values suggested that loci F13A01, FES/FPS, F13B and LPL are discriminative and very useful to solve complex forensic casework, and should be added to the set of STRs loci routinely used in forensic laboratories. In conclusion, an additional 4 loci dataset was established for the south Portuguese population, which can be used for both forensic casework and in complex kinship testing.