Abstract
The short tandem repeat (STR) loci are polymorphic markers in the combined DNA index system (CODIS) and non-CODIS STR loci. Due to the highly polymorphic characteristic of STR loci, they are popular and widely used in forensic DNA typing laboratories. In this study, 22 STR loci (1 CODIS, 21 non-CODIS STR loci) and an Amelogenin locus were genotyped and analyzed in 590 unrelated individuals of the Guanzhong Han population. None of the 22 STR loci deviated from the Hardy–Weinberg equilibrium, and all the loci were in the linkage equilibrium state. We observed 247 alleles, and the corresponding allelic frequencies ranged from 0.0008 to 0.3695 in the Guanzhong Han population. The combined power of discrimination and the cumulative exclusion probability was 0.999 999 999 999 999 999 999 999 999 346 36 and 0.999 999 999 709 74, respectively. The results including Nei's DA genetic distance, multidimensional scaling analysis, and principal component analysis showed that the Guanzhong Han population has closer genetic affinities with Northern Han, Chengdu Han, and Xinjiang Hui groups from China based on allelic frequencies of 15 overlapped STR loci from Guanzhong Han and 13 reference groups. The present results indicated that Microreader™ 23sp ID kit included highly polymorphic loci, and it could be well used for individual identification, paternity testing, and population genetics in the Guanzhong Han population.
Highlights
Short tandem repeats (STRs), as the molecular genetic marker of DNA length polymorphism, are widely spread in the human genome and often used to study population genetics, individual identification, and paternity testing by forensic researchers [1, 2]
P values of the Linkage disequilibrium (LD) tests were presented in Table S2, and the results showed that the P values of 25 pairs of 231 pairs in 22 short tandem repeat (STR) loci were less than 0.05
There were no significant deviations from Hardy–Weinberg equilibrium (HWE) (P ≤ 0:05/22) and linkage equilibrium (P ≤ 0:05/231) after using Bonferroni correction, indicating that these loci were independent of each other and could be performed in subsequent calculations using the multiplication rule
Summary
Short tandem repeats (STRs), as the molecular genetic marker of DNA length polymorphism, are widely spread in the human genome and often used to study population genetics, individual identification, and paternity testing by forensic researchers [1, 2]. In order to improving the power of identification and reducing the probability of mismatching, seven polymorphic STR loci have been added to 13 CODIS STR loci, turning it to 20 CODIS STR loci [9]. It did not meet the purpose of forensic researchers sometimes. The combined CODIS STR loci and non-CODIS STR loci can improve the BioMed Research International efficiency of individual identification of the whole system and improve the performance of paternity testing
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