Population genetic comparisons of three X-chromosomal STRs (DXS7132, DXS7133 and GATA172D05) in North and South Italy

Abstract

Population genetic data of three tetranucleotide X-chromosomal STRs, DXS7132, DXS7133 and GATA172D05, were obtained by analyzing 295 unrelated healthy individuals living in North and South Italy (160 females and 135 males), and 40 family trios with female children. PCR primers for the loci DXS7132 and DXS7133 were redesigned in order to reduce the length of the amplification products compared with conventional design so that improved typing success rate when highly degraded DNA is used as a template. The comparison of the allele frequencies of these three ChrX markers gave similar distributions for North and South Italy although minor variations were found for some alleles. Additionally, some differences were found when comparing the allele frequencies of the male and female samples independently. Based on the investigated meiotic events, mutations were not found.