Poorly conductive biochar boosting extracellular electron transfer for efficient volatile fatty acids oxidation via redox-mediated mechanism

Abstract

This study explored the performances, and associated mechanisms of biochar promoting volatile fatty acids (VFA) oxidation via extracellular electron transfer (EET) pathway. It was found that in a bioelectrochemical system, adding biochar suspension remarkably enhanced electricity generation whatever acetate or propionate used as an electron donor. The maximum current density in biochar-assisted groups reached 1.6–2.2 A/m2, which were 69.2–220.0% higher than that of control groups. The lower electrical resistance of anode in biochar-assisted groups was potentially attributed to the formed biofilm dominated by electro-active Geobacteraceae, and the electron donor type depending on dominant genus. In specific, with biochar assistance, Desulfuromonas enriched from 1.1% to 25.0% when acetate as an electron donor, and the relative abundance of Geobacter increased from 4.6% to 31.7% as dominant genus in propionate-added group. Electrochemical analysis uncovered that biochar hardly elevated sludge electrical conductivity, while the excellent redox-based electron exchange transfer capacity likely made biochar as a transient electron acceptor, which was more accessible than anode to support the metabolism of electroactive bacteria in the initial stage. Meanwhile, the porous surface area of biochar particle likely provided a “bridge” between suspended sludge and anode, to support a more directional evolution of electroactive bacteria on anode. This dual-function of biochar achieved a sustainable VFA oxidation via EET-based pathway.