Abstract

In the study nasal and throat swabs were collected from 78 apparently healthy and 69 cases of kennel cough from dogs of different age, sex, breed and places and analysed for presence of Bordetella bronchiseptica through bacterial isolation and multiplex PCR (mPCR). The pathogen could be isolated from an apparently healthy Labrador bitch while with mPCR, 7 (4.76%, 4 from healthy dogs and 3 from sick dogs) samples were positive for genus specific amplicon and three (2.04%, one from healthy and two from sick Labrador bitches) were positive for B. bronchiseptica species specific amplicons. The kennel cough had no significant association (p, 0.49) with the detection of B. bronchiseptica but sex (p, 0.03) appeared to be the significant predisposing factors associated with the infection.

Highlights

  • Contagious respiratory tract infections in dogs are commonly diagnosed as canine infectious tracheobronchitis or kennel cough

  • With multiplex polymerase chain reaction (PCR) (mPCR), 7 (4.76%, 4 from healthy dogs and 3 from sick dogs) samples were positive for genus specific amplicon and three (2.04%, one from healthy and two from sick dogs Labrador bitches) were positive for B. bronchiseptica specific amplicons (Fig. 3)

  • All the dogs which were positive for B. bronchiseptica either through isolation or through mPCR were all Labrador bitches revealing a significantly more proneness of females to B. bronchiseptica colonization (p, 0.03) with respect to isolation or detection of pathogen with genus specific PCR no such difference was apparent

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Summary

Introduction

Contagious respiratory tract infections in dogs are commonly diagnosed as canine infectious tracheobronchitis or kennel cough. Kennel cough affects dogs of all ages and breeds [1, 2]. Of the several pathogens associated with kennel cough Bordetella bronchiseptica is considered to be the most important one [3, 4]. Bordetella bronchiseptica, a zoonotic respiratory pathogen, causes several other disease syndromes in human and other animals [5, 6]. It is world-wide in occurrence but its status is little understood in India [7, 8]. To understand the association of B. bronchiseptica with kennel cough in dogs this pilot study was undertaken

Materials and Methods
Results and Discussion
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