Abstract
Sections of Taenia hydatigena cyst membranes stained with periodic acid-Schiff reagent showed intense staining of the tegument, the reticular fibers, and of many irregularly dispersed globules of various sizes. In sections treated with malt diastase, only the tegument and reticular fibers stained. A product obtained from cyst membranes by cold water extraction closely resembled a reference glycogen. Protein could not be detected in this product and glucose was the only monosaccharide noted following hydrolysis and paper chromatography. Upon ultracentrifugation of this product, two peaks were obtained with calculated sedimentation coefficients ( S 20 w ) of 184S and 919S. Membranes treated with NaOH yielded, upon precipitation with ethyl alcohol, a product with an infrared spectrum closely resembling the reference glycogen. During electrophoresis on paper this material migrated as a single band. Precipitation with acetone of the supernatant from the above NaOH-treated membrane gave a product with characteristics of a mucopolysaccharide. This substance was unaffected by diastase digestion and yielded, upon hydrolysis, glucose and 10–15 amino acids. During electrophoresis on paper most of this material remained at the point of application. On further purification and hydrolysis of this material, glucose, glucosamine, galactosamine, and 14 amino acids were detected.
Published Version
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