Polypyrrole modified stainless steel frits for on-line micro solid phase extraction of ochratoxin A

Abstract

Polypyrrole (PPy) was electrochemically synthesized on stainless steel frits as a sorbent for the micro solid phase extraction (muSPE) of ochratoxin A (OTA). Using 20 microl of standard solution under a fast flow rate of 0.5 ml/min, 80% recovery of OTA was achieved in the concentration range from 0.1-10 pg/mul. This good recovery was achieved within a short residence time of 1.2 s. A binding capacity of 1 ng OTA was estimated for each PPy-modified frit, or 2 ng OTA for two frits in series. The bound OTA could be pulsed eluted (PE) with 20 microl of 1% triethylamine in acetonitrile. On-line coupling of this PPy-on-a-frit and PE technique to high performance liquid chromatography (HPLC) was straightforward. On-line muSPE-PE-HPLC results clearly demonstrated the capability of PPy-on-a-frit to bind OTA in the presence of red wine, beer, and orange juice components.