Polypyrrole electrospotting for the construction of oligonucleotide arrays compatible with a surface plasmon resonance hybridization detection

Abstract

We describe in this paper a new electrospotting process allowing a straightforward functionalization of homogeneous gold surfaces. It is based on a direct electro-copolymerization of pyrrole and pyrrole linked to an oligonucleotide on a gold substrate via the use of a moving micro electrochemical cell. The polypyrrole spots obtained by this way were characterized in terms of thickness and DNA hybridization efficiency. Results show that either fluorescence or surface plasmon resonance detection of DNA hybridization can be carried out on these substrates.