Polypyrrole as an Efficient Solid-Phase Extraction Sorbent for Determination of Chloramphenicol Residue in Chicken Liver, Kidney, and Meat

Abstract

A novel, sensitive, inexpensive, and efficient method for the extraction and pre-concentration of chloramphenicol residue in chicken tissues has been developed. In this work, polypyrrole polymer was synthesized and used as an efficient sorbent for solid-phase extraction. Several parameters such as type of salt, salting out effect, pH of extraction solution, temperature, and desorption solvent were optimized. The samples were analyzed by high performance liquid chromatography coupled to photodiode array detection. Under optimized extraction and cleanup conditions (namely, Britton–Robinson buffer 0.1 M adjusted to pH 2.0 with NaCl at 35 °C and using of methanol as desorption solvent), the method showed good linearity and sensitivity. Recovery was in the range of 86.0–93.4, 80.8–89.5, and 77.5–85.6 % for meat, kidney, and liver, respectively. Relative standard deviation was lower than 10 % in all cases indicating that the method can be used as a validated method. The limit of detection and limit of quantitation of the proposed method at various matrices were found to be in the range of 0.1–1 and 0.3–3 ng/g, respectively. These results suggested that the solid-phase extraction base on polypyrrole might be a potential candidate for the extraction of chloramphenicol from chicken tissues.