Polyphenolics in ramontchi protect cardiac tissues via suppressing isoprenaline-induced oxidative stress and inflammatory responses in Long-Evans rats

Abstract

• HPLC analysis revealed the presence of potent polyphenolics in ramontchi. • Polyphenolics in ramontchi impede cardiac damages in Long-Evans rats. • Ramontchi normalized expressions of antioxidant genes i.e. , Nrf-2, HO-1, HO-2, GPx, SOD and CAT. • Ramontchi suppressed the expressions of inflammatory mediators i.e. , IL-1, IL-6, TNF-α, TGF-β1, iNOS and NF-κB. • Ramontchi diminished the level of oxidative stress in isoprenaline-induced rats. This investigation was aimed to explore the cardioprotective effect of Ramontchi fruits ( Flacourtia indica ; FI) in isoprenaline-administered Long-Evans rats. Administration of isoprenaline increased the total heart wet weight, MDA, NO, and APOP as well as ALT, AST, and CK-MB levels, which were normalized by FI fruits treatment. Moreover, expressions of antioxidant genes i.e. , Nrf-2, HO-1, HO-2, SOD, catalase, and GPx were normalized by FI fruits in isoprenaline-induced rats. Besides, expression of inflammatory mediators i.e. , IL-1, IL-6, TNF-α, TGF-β, iNOS, and NF-κB were also suppressed by FI fruits. The outcome of FI fruits treatment was noted as promising and comparable to the standard atenolol treatment. The enhanced biological activity shown by FI fruits may be due to its abundant polyphenolic compounds as determined by HPLC analysis. In conclusion, FI fruits may protect cardiac tissue in isoprenaline-administered rats by suppressing the oxidative stress and proinflammatory mediators and enhancing the antioxidant enzyme functions.