Abstract
The aim of this study is to characterize the antioxidant capacity and establish the profile of polyphenolic compounds in walnut extracts (different extracts prepared from walnut leaf and green husks). The correlation between bioingredients of the product tested and their ability to scavenge free radicals and reduce them by chelating various metal ions were examined. Research technology combining TG (thermogravimetry), FTIR (Fourier-transform infrared spectroscopy), high-performance liquid chromatography system (HPLC) with electrochemical methods (cyclic and differential pulse voltammetry) and spectrophotometric methods (ABTS, FRAP, and DPPH assays) was used to rate the potential oxidation-reduction components of walnut extracts. A high affinity for scavenging free radicals ABTS and DPPH was found for natural substances present in leaves and green husks. The walnut is beneficial to health as it contains alpha-linolenic acid in its lipid fraction and, as demonstrated in this study, its husks are rich in polyphenolics with high antioxidant capacity.
Highlights
Natural polyphenols are a large group of secondary plant metabolites, arising from phenylalanine or shikimic acid
The aim of this work was to determine the antioxidant capacity of leaf and walnut green husk extracts by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV)
CV and DPV electrochemical methods were applied to determine the antioxidant capacity of leaf extracts and walnut husks
Summary
Natural polyphenols are a large group of secondary plant metabolites, arising from phenylalanine or shikimic acid. Natural polyphenols affect the organoleptic properties of plants and foods of plant origin [1] These compounds are well-known for their advantageous effects on human health. The literature describes their antioxidant, cardioprotective, cytotoxic, anti-inflammatory, as well as antibacterial properties [2,3]. As they are widely found in plants, these substances are a beneficial alternatives to antibiotics and additives of chemical origin. Plant materials (leaves, bark, unripe nuts) obtained from this tree was commonly used in folk medicine for its strong antioxidant capacity, antidiabetic, antibacterial, anti-inflammatory, anti-atherogenic, and liver-protective properties [4,5].
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