Abstract
By treatment of avian myeloblastosis virus (AMV) with NP 40, it was possible to quantitatively isolate a rosettelike structure whose subunits are related to the surface projections of the virus. Detailed analysis of this material resulted in the following findings: (i) The rosettes represented about 7% of the protein and 60% of the carbohydrate content of the virus. (ii) SDS-polyacrylamide gel electrophoresis indicated the presence of two glycoproteins having molecular weights of 37,000 (GI) and 115,000 (GII). (iii) GII could be isolated without treatment with SDS and structurally resembled the knob portion of the rosettes. (iv) Both GI and GII specifically absorbed neutralizing sera and precipitated with these in immunodiffusion analysis. (v) In addition to type-specific antigenicity, two distinct antigenic determinants were demonstrated in the rosettes utilizing nonneutralizing rabbit sera. One of these resided in GI and the other in GII. These antigens were shown to be distinct from the major complement-fixing antigens of the virus. These results confirm the belief that the surface projections of avian RNA tumor viruses represent the type-specific antigens.
Published Version
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