Abstract
Chemical investigation of the ethanol extract of soft coral Sinularia sp. collected from the South China Sea led to the isolation of three new polyoxygenated sterols, (3S,23R,24S)-ergost-5-ene-3β,23α,25-triol (1), (24S)-ergostane-6-acetate-3β,5α,6β,25-tetraol (2), (24S)-ergostane-6-acetate-3β,6β,12β,25-tetraol (3) together with three known ones (4–6). The structures, including relative configurations of the new compounds (1–3), were elucidated by detailed analysis of spectroscopic data (IR, UV, NMR, MS) and by comparison with related reported compounds. The absolute configuration of 1 was further determined by modified Mosher’s method. Compound 5 exhibited moderate cytotoxicity against K562 cell line with an IC50 value of 3.18 μM, but also displayed strong lethality toward the brine shrimp Artemia salina with a LC50 value of 0.96 μM.
Highlights
Soft coral of the genus Sinularia has been found to be a rich source of bioactive secondary metabolites [1,2], such as acylated spermidine [3,4], lipids and fatty acids [5], cyclic sesquiterpeneMar
The test of brine shrimp toxicity on A. salina was performed according to standard protocols [28,29]
South China Sea, this study provided a series of polyoxygenated sterols
Summary
Soft coral of the genus Sinularia has been found to be a rich source of bioactive secondary metabolites [1,2], such as acylated spermidine [3,4], lipids and fatty acids [5], cyclic sesquiterpene. As part of our ongoing investigation of new natural bioactive compounds from marine invertebrates in the South China Sea [11,12,13,14,15,16], the soft coral Sinularia sp. Attracted our attention because the crude extract of Sinularia sp. Showed lethal activity toward brine shrimp Artemia salina. Bioassay-guided fractionation of the active extracts led to the isolation of three new polyoxygenated sterols (1–3) and three known ones (4–6) [17,18] (Figure 1)
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