Abstract
Four methanol-utilizing yeasts, Candida boidinii, Hansenula polymorpha, Hansenula ofunaensis, and Pichia pinus, produced polyols from corresponding sugars in a methanol medium. H. polymorpha produced larger amounts of xylitol than the other yeasts. Productivity was the highest at pH 8 when 5 g (dry)/ l cultured cells were incubated with 2.5 g/ l urea as the nitrogen source in a medium containing 1% (v/v) methanol and 1 g/ l MgSO 4·7H 2O. Under these conditions, 57 g/ l xylitol was obtained from 110 g/ l d-xylose after 3 d of cultivation. The largest amount of xylitol (58 g/ l; yield, 0.62 g/g) was produced from 125 g/ l, d-xylose and 5% (w/v) glycerol instead of methanol after 4 d of cultivation.
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