Abstract
Methylotrophic yeasts showed 3 different patterns in regard to the initial step of glycerol dissimilation. Hansenula polymorpha DL-1 had activities of NAD+-linked glycerol dehydrogenase (GDH) and glycerol kinase (GK), which were induced by glycerol and methanol and by glycerol, respectively. Hansenula ofunaensis had the GDH activity, which was induced by glycerol but not by methanol. Candida boidinii No. 2201 had the GK activity, which was induced by glycerol. It was concluded that the phosphorylative pathway in C. boidinii No. 2201, the oxidative pathway in H. ofunaensis, and both the pathways in H. polymorpha DL-1 may be essential for glycerol dissimilation.
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