Polymorphisms in miR-135a-2, miR-219-2 and miR-211 as well as their interaction with cooking oil fume exposure on the risk of lung cancer in Chinese nonsmoking females: a case-control study.

Hang Li,Biyun Qian,Baosen Zhou,Zhihua Yin,Yangwu Ren,Qing Lan,Nathaniel Rothman,Zhigang Cui

doi:10.1186/s12885-016-2784-1

Hang Li, Biyun Qian + Show 6 more

Open Access

https://doi.org/10.1186/s12885-016-2784-1

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Abstract

BackgroundThe associations between microRNAs and lung cancer have received increasing attention. This study assess the association between polymorphisms in miR-135a-2, miR-219-2 and miR-211 genes and the risk of lung cancer, as well as the gene–environment interaction between these polymorphisms and cooking oil fume exposure.MethodsA case–control study featuring 268 cases and 266 controls was conducted. The associations of miR-135a-2 rs10459194, miR-219-2 rs10988341 and miR-211 rs1514035 polymorphisms with the risk of lung cancer were analyzed. The gene–environment interactions were also reported on both additive and multiplicative scales.ResultsThere were no statistically significant associations between the single-nucleotide polymorphisms (SNPs) and lung cancer or lung adenocarcinoma. The individuals with both a risk genotype of miRNA SNPs and exposure to a risk factor (cooking oil fumes) were at higher risk of lung cancer than those with only one of these two risk factors (odd ratios of 2.208, 1.285 and 1.813 for miR-135a-2 rs10459194; 2.164, 1.209 and 1.806 for miR-219-2 rs10988341; and 2.122, 1.146 and 1.725 for miR-211 rs1514035, respectively). However, the measures of biological interaction indicate that there was no such interaction between the three SNPs and exposure to cooking oil fumes on an additive scale. Logistic regression models also suggested that the gene–environment interactions were not statistically significant on a multiplicative scale.ConclusionsThere were no significant associations between the polymorphisms in miRNAs (miR-26a-1 rs7372209, miR-605 rs2043556 and miR-16-1 rs1022960) and the risk of lung cancer in the Chinese nonsmoking female population. The interactions between these polymorphisms in miRNAs and cooking oil fume exposure were also not statistically significant.

Highlights

The associations between microRNAs and lung cancer have received increasing attention
This study was approved by the Institutional Review Board of China Medical University and written informed consent was obtained from each participant
The numbers of cases and controls with a history of cooking oil fume exposure were 100 (37.3 %) and 66 (24.8 %); the incidence of exposure was higher in cases than in controls (χ2 = 9.739, P = 0.002)

Summary

Introduction

The associations between microRNAs and lung cancer have received increasing attention. This study assess the association between polymorphisms in miR-135a-2, miR-219-2 and miR-211 genes and the risk of lung cancer, as well as the gene–environment interaction between these polymorphisms and cooking oil fume exposure. MicroRNA (miRNA) is a class of highly evolutionarily conserved noncoding RNA 18–25 nucleotides in length, which accounts for 1–5 % of the human genome [4] and regulates the expression of approximately >60 % of protein-coding genes. It has been suggested that a single miRNA can influence the expression of a variety of cancer-related genes. A difference in the expression level of miRNA between cancerous tissue and adjacent healthy tissue was observed in previous studies, suggesting that miRNA plays a role in tumorigenesis as a tumor suppressor or oncogene, depending on the context

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