Abstract
ObjectiveTo analyze mitochondrial DNA (mtDNA) polymorphisms in coding area and provide a theoretical basis to apply in Forensic Science. MethodsThe primers of 8162F/8483R and 13070F/13299R were designed according to the Anderson's sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162–8483 and nt13070–13299. ResultsThe lengths of the amplicons were 322 bp and 230 bp, respectively. There were 24 mitochondrial haplotypes defined by 21 variable positions in both regions. The gene diversity was estimated at 75.11%, and the probability of two randomly selected individuals having identical mtDNA types was 25.64%. ConclusionsThe polymorphic sites within mtDNA coding area can be useful in combination with mtDNA control region in order to increase the discriminational power.
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