Abstract
Objective: This work aimed to clarify the association of the severity of periodontitis and polymorphism in osteoprotegerin (OPG) (T950C) inIndonesian men.Methods: For DNA extraction, blood serum samples were used from 100 consenting Indonesian males for whom also the status of periodontitis hadbeen classified as mild, moderate, or severe. Polymerase chain reaction and restriction fragment length polymorphism techniques were applied toevaluate OPG (T950C) polymorphism, using Hind II restriction enzyme and electrophoresis on agarose gel to separate the indicative fragments.Results: The genotype distribution of the OPG (T950C) polymorphism had an appearance of an increasing percentage of TT genotype (allele T) withincreasing severitPeriodontitisy of periodontitis. The CC genotype was relatively rare (1%) in the tested Indonesian male population.Conclusions: The results show no significant association between the severity of periodontitis and polymorphism of OPG (T950C) in Indonesian men.
Highlights
Periodontitis is a complex multifactorial disease with high prevalence in Indonesia and in the world
50% of the population aged 16–80 years in Denmark had a healthy periodontium and 50% suffered from gingivitis and periodontitis in 1981
Every person has an individual body response that will determine the susceptibility to periodontitis. The variants such as polymorphisms of the key genes controlling the host response in periodontitis may affect the susceptibility to the disease [9]
Summary
For DNA extraction, blood serum samples were used from 100 consenting Indonesian males for whom the status of periodontitis had been classified as mild, moderate, or severe. Polymerase chain reaction and restriction fragment length polymorphism techniques were applied to evaluate OPG (T950C) polymorphism, using Hind II restriction enzyme and electrophoresis on agarose gel to separate the indicative fragments
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